Part:BBa_K389004:Design

Luciferase from pGL4.10[luc2]

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 64
  Illegal NgoMIV site found at 1408
  Illegal NgoMIV site found at 1429
  Illegal AgeI site found at 1132
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1314
  

Design Notes

sequence of this vector is already published by Promega [http://www.promega.com/vectors/pgl4.10.txt here]

Source

pGL4.10[luc2]

References

• Conti E, Franks NP, Brick P (1996) [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VSR-4CJB7RR-7&_user=2459438&_coverDate=03%2F31%2F1996&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_acct=C000057302&_version=1&_urlVersion=0&_userid=2459438&md5=ac60aa60eaad17b2aa1c4b31f954d0a8&searchtype=a Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes], Structure, 4(3):287-98.

• wikipedia article: http://en.wikipedia.org/wiki/Luciferase

• Sequence of pGL4.10[luc2]: http://www.promega.com/vectors/pgl4.10.txt